Microscopy product ID: 61937
2D Image
Description | Zero degree tilt image of a presynaptic bouton from a cultured neuron expressing alpha synuclein-MiniSOG processed for photo-oxidation. Extensive stained membranous elements are observed in the pre-synaptic terminal. Data shown in Fig. 4B of Boassa et al., 2013. Use the brightness and contrast controls to adjust image. |
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Segmentation
Description | Manual segmentation of plasma membrane and labeled tubero-membranous system in a synaptic terminal |
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Description | Three-dimensional model from EM tomogram of same area as 4B shows plasma membrane (pink) and contiguous membranes in three different colors (blue, green, and yellow). |
Reconstruction
Description | Computed slices through a double tilt electron tomographic reconstruction of synaptic terminals in rat cultured cortical neurons expressing alpha synuclein-MiniSOG and processed for photo-oxidation. Extensive stained membranous elements are observed in the pre-synaptic terminal Data corresponds to Fig. 4 in Boassa et al. 2013. |
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Description | Computed slices through a double tilt electron tomographic reconstruction of synaptic terminals in rat cultured cortical neurons expressing alpha synuclein-MiniSOG and processed for photo-oxidation. Extensive stained membranous elements are observed in the pre-synaptic terminal Data corresponds to Fig. 4 (Tomogram of cultured neurons expressing AS-MiniSOG processed for photooxidation. Extensive stained membranous elements are observed in the presynaptic terminal.)in Boassa et al. 2013. |
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Boassa, Daniela; Berlanga, Monica L.; Yang, Mary Ann; Terada, Masako; Hu, Junru; Bushong, Eric A.; Hwang, Minju; Masliah, Eliezer; George, Julia M.; Ellisman, Mark H. (2017). Microscopy product ID: 61937. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0M908GQ
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- Experiment: 2011-03-08.
- Date Issued
- 2017
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- Technical Details
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Product type: DOUBLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000EX
Strain: Sprague Dawley
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- Anatomy
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- Related Resources
- Boassa D, Berlanga ML, Yang MA, Terada M, Hu J, Bushong EA, Hwang M, Masliah E, George JM, Ellisman MH (2013). Mapping the subcellular distribution of alpha-synuclein in neurons using genetically encoded probes for correlated light and electron microscopy: Implications for Parkinson's disease pathogenesis. J Neurosci, 33(6):2605-2615. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3711410/; Journal of Neuroscience. https://doi.org/10.1523/JNEUROSCI.2898-12.2013
- Microscopy product 61937 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB61937
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2023-05-22