Allen, Richard (2021). CIL:12031, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05X281T

A high resolution image of a tangential section through the cell's surface show the pairs of basal bodies in cross section. The ends of kinetodesmal fibers point anteriorly in the cell, the postciliary ribbons arise from the posterior right side of the posterior basal body of a pair and transverse ribbons arise from the left side of both basal bodies of a pair. The more anterior basal body of a pair is linked to the posterior basal body by a striated fiber and to the adjacent kinetodesmal fiber by a short filamentous connection (arrowhead). Parasomal sacs, infraciliary lattice, striated band, and trichocyst tips are evident. TEM taken on 1/12/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,900X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 3957

• Paramecium multimicronucleatum

• Cell by organism
• Cell cortex
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Kinetodesmal fiber
• Microtubule
• Microtubule basal body
• Other fiber systems
• Trichocyst

• Cortical cytoskeleton organization
• Microtubule basal body organization
• Microtubule cytoskeleton organization
• Trichocyst organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12031

Doi: https://doi.org/10.6075/J05X281T

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12031

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2022-08-12