Ladinsky, Mark S.; McIntosh, J. Richard; Mastronarde, David N.; Howell, Kathryn E.; Staehelin, L. Andrew (2021). CIL:24496, Rattus norvegicus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RN370V

This image is from a tomogram through part of a Golgi ribbon from a normal rat kidney cell, generated using high voltage electron microscopy following incubation at 15°C to block transport out of the Golgi complex. Following temperature block, large bulging domains appear on the three trans-most cisternae. The sample was tilted at angles of 1.5° to obtain the series of images in this set, and used to obtain 3-dimensional reconstructions of the Golgi apparatus and contributed to findings reported in Ladinsky et al. (1999) Golgi structure in three dimensions: Functional insights from the normal rat kidney cell. J. Cell Biol. 144 (6) 1135-1149.

• 2021

• Howell, Kathryn E.
• Ladinsky, Mark S.
• Mastronarde, David N.
• McIntosh, J. Richard
• Staehelin, L. Andrew

Cells were grown on 100-mesh gold EM grids, and maintained at 15°C for 4 hours before plunge freezing in liquid nitrogen (BalTec HPM-010), followed by freeze-substitution (1% glutaraldehyde, 0.1% tannic acid in acetone, replaced with 4% osmium tetroxide and 0.01% uranyl acetate), then embedded in Epon-Araldite and sectioned at 250nm (UltraCut-UCT, Leica). Sections were transferred to formvar-coated copper-rhodium slot grids (EMS) and stained with 2% aqueous uranyl acetate and Reynold's lead citrate. Digital images were acquired at a magnification of 15,000X with an FEI Tecnai TF20 transmission EM. For additional details refer to: J. Cell Biol. 144 (6) 1135-1149.

Preparation: tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data
Data qualification: Raw

• Cell Image Library Group ID: 8574

• Rattus norvegicus

• Epithelial cell
• Golgi apparatus
• Golgi cisterna

• Golgi organization
• Golgi vesicle budding
• NRK-52E

• data
• image

• No linguistic content; Not applicable

Samplenumber: 24496

Doi: https://doi.org/10.6075/J0RN370V

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL24496

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2022-08-12