Ris, Hans (2021). CIL:9531, notopthalmus viridescens, erythrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0B56HTH

Newt (Notopthalmus viridescens) erythrocytes were isolated in 0.1M KCl, spread on the surface of 5mM Na-citrate, picked up on formvar-carbon films, fixed with glutaraldehyde and paraformaldehyde and negatively stained with sodium tetraborate. Images were recorded at 40KX and accelerating voltage of 1MeV at the University of Wisconsin HVEM facility.This image was taken with a specimen tilt of 55 degrees. A grouped image of the same field at a 45 degree tilt provides an oblique stereo view of the tangle of chromatin fibers emanating from the dispersed nucleus.

• 2021

• Ris, Hans

Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue
Relation to intact cell: spread nucleus
Item type: film
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: other negative stain
Processing history: unprocessed raw data

• Cell Image Library Group ID: 2464

• Notopthalmus viridescens

• Erythrocyte
• Nuclear chromatin

• DNA packaging

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9531

Doi: https://doi.org/10.6075/J0B56HTH

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9531

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=7022124
• Ris H. Stereoscopic electron microscopy of chromosomes. Methods Cell Biol. 1981;22:77-96. https://doi.org/10.1016/s0091-679x(08)61871-3

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2022-08-12