Horowitz-Scherer, Rachel; Woodcock, Christopher (2021). CIL:763, Patiria miniata, sperm. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0SQ8ZNZ

Upon electron bombardment, some elements, such as P, show an electron energy loss profile with a peak at a specific energy loss value. By collecting a series of images of a field at different energy losses, it is possible to compute the relative amount of the element at each pixel and map the element's distribution, a technique known as electron spectroscopic imaging (ESI) or electron filtered transmission EM (EFTEM).
The 4 images in this group were recorded from unstained <30 nm section with 5eV energy windows centered on 120, 104, 160 and 170 eV respectively using a Zeiss 902 energy filtered EM, making it possible to map the distribution of phosphorus (energy loss edge 160 eV) within the chromatin fibers. This image was recorded at 1o4 eV.

• 2021

• Horowitz-Scherer, Rachel
• Woodcock, Christopher

Preparation: glutaraldehyde fixed tissue; freeze substitution in Lowicryl
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: microscopy with lenses; transmission electron microscopy (TEM)
Parameter imaged: modes of electron microscopy; EELS imaging
Source of contrast: electron scattering
Visualization methods: electron energy loss window
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8090

• Patiria miniata

• Chromatin
• Sperm

• DNA compaction
• Reproduction

• data
• image

• No linguistic content; Not applicable

Samplenumber: 763

Doi: https://doi.org/10.6075/J0SQ8ZNZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL763

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12