CIL:36657, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:36657, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GQ6WRX
- Description
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During self conjugation two cells from a 6-day old culture come together, bridges are formed between them and haploid products of meiotic division of their micronuclei are exchanged between the mating pairs. Two micronuclei are seen here which appear similar to the haploid pronucleus in the paper of Inaba et al. (Proc. Japan Acad. 42:394-398, 1966) which is passing through such a bridge in P. multimicronucleatum. In this micrograph two rounded micronuclei are lying very near the macronucleus. These may be the migratory and stationary pronuclei that will form the diploid synkaryon nucleus from which the new micronuclei and macronucleus will form after further mitotic divisions. Interestingly one of these nuclei appears to be in close contact with the macronucleus. TEM taken on 3/4/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 5,000X. Bar = 1µm.
- Date Issued
- 2021
- Researcher
- Methods
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Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed - Scientific Name
- Anatomy
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 36657
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36657
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12