CIL:717, Necturus maculosus, nucleate erythrocyte
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- Cite This Work
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Woodcock, Christopher (2021). CIL:717, Necturus maculosus, nucleate erythrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0DV1J6G
- Description
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X-ray analysis shows that the nucleated erythrocytes of the amphibian Necturus maculosus (Mud Puppy) contains more ordered chromatin than most nuclei. Here, chromatin fibers were isolated from N. maculosus erythrocyte nuclei using mild micrococcal nuclease digestion, fixed briefly with glutaraldehyde, applied to thin carbon films and negatively stained with aurothioglucose. ~30 nm fibers are seen, some exhibiting short regions with apparent structural order.
- Date Issued
- 2021
- Researcher
- Technical Details
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Preparation: unembedded tissue; glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: elastic scattering
Visualization methods: other negative stain
Processing history: raw unprocessed data - Scientific Name
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- Identifier
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Samplenumber: 717
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL717
- PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=1757505
- Woodcock CL, Woodcock H, Horowitz RA. Ultrastructure of chromatin. I. Negative staining of isolated fibers. J Cell Sci. 1991 May;99 ( Pt 1):99-106. https://doi.org/10.1242/jcs.99.1.99
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12