CIL:40248, Canis lupus familiaris, epithelial cell
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- Collection
- Cite This Work
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Boassa, Daniela (2021). CIL:40248, Canis lupus familiaris, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01V5F30
- Description
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Maximum intensity projection of a confocal z series of MDCK cells expressing Cx43-GFP-4C imaged with confocal microscopy. This image has been downsampled from the raw data image, and time lapse series, both of which can be accessed using the link provided to the Cell Centered Database. For more information, see: Boassa et al. (2010) Trafficking and Recycling of the Connexin43 Gap Junction Protein during Mitosis. Traffic. PMID: 20716111.
- Date Issued
- 2021
- Researcher
- Methods
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Madin-Darby canine kidney (MDCK) cells were maintained at 37° C, and 10% CO2 in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (FBS) (Gibco-BRL, Invitrogen). Transductions were carried out using a retroviral system according to the protocols from the Nolan laboratory (www.stanford.edu/group/nolan). Experiments were conducted on endogenous expressing or stably Cx43-expressing cell lines generated by transduction followed by selection with the antibiotic hygromycin (Gibco-BRL, Invitrogen). In order to image several cells progressing through mitosis, cells were synchronized using a combination of serum starvation and application of aphidicolin (APD), an inhibitor of DNA synthesis, to create an enriched population of cells undergoing mitosis. Confluent cells were trypsinized and grown for about 20 h in the presence of minimal serum and APD, resulting in a G1/S block. The drug was then washed out, normal serum was restored and the cells were allowed to progress through S phase to mitosis. Samples were mounted in Opti-MEM and time-lapse imaging of the intrinsic GFP was performed after 7 h, when cells began entering mitosis, using an Olympus Fluoview 1000 with a 60X NA 1.42 objective.
- Technical Details
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Relation to intact cell: living tissue; dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Data qualification: Processed - Series
- Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 40248
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40248
- Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0DB81NK
Source data
Previous version
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12