Shlyaktenko, Luda; Woodcock, Christopher (2021). CIL:7407, Homo sapiens, neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0DN4437

The protein MeCP2 binds to symmetrically methylated CpG dinucleotides and is thought to transmit the (generally) repressive signals encoded in DNA methylation patterns. Mutations in MeCP2 lead to Rett syndrome. Here, MeCP2-DNA complexes have been imaged by AFM (Digital Instruments Nanoscope) in air using human MeCP2 and an 828 bp DNA sequence containing several methylated CpGs. MeCP2, which appears as bright circles in AFM images, and volume measurements of the complexes suggest that MeCP2 monomers and dimers are present and that MeCP2 binds DNA cooperatively. Other images in the linked set show looped structures and large complexes containing multiple DNA strands and MeCP2 molecules. These configurations are likely promoted by the multiple independent DNA-binding domains of MeCP2.

• 2021

• Shlyaktenko, Luda
• Woodcock, Christopher

Preparation: glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: microscopy; atomic force microscopy
Parameter imaged: specimen height
Source of contrast: specimen height
Processing history: Pixel value determine by specimen height

• Cell Image Library Group ID: 7760

• Homo sapiens

• Neuron
• Nucleus

• Rett syndrome
• Transcription regulation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7407

Doi: https://doi.org/10.6075/J0DN4437

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7407

Reference

• Ghosh RP, Horowitz-Scherer RA, Nikitina T, Shlyakhtenko LS, Woodcock CL. MeCP2 binds cooperatively to its substrate and competes with histone H1 for chromatin binding sites. Mol Cell Biol. 2010 Oct;30(19):4656-70. https://doi.org/10.1128/MCB.00379-10
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=20679481

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2022-08-12