Bushong, Eric A.; Ellisman, Mark H.; Gonzales, Cesar Nava; Madany, Matthew; McKaughan, Quintyn; Su, Chih-Ying; Tsang, Tin Ki (2024). CIL: 54607. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MK6CQ4

The biophysical properties of sensory neurons are influenced by their morphometric and morphological features, whose precise measurements require high-quality volume electron microscopy (EM). However, systematic surveys of these nanoscale characteristics for identified neurons are scarce. Taking advantage of the CryoChem method, which permits high-quality ultrastructural preservation of cryofixed and genetically labeled samples for volume EM, we acquired serial block-face scanning electron microscopy (SBEM) images of antennal tissues in which select ORNs expressed the APEX2 EM marker. These SEBEM datasets allow a systematic morphometric and morphological analysis of identified olfactory receptor neurons in Drosophila melanogaster.

• 2024

• Bushong, Eric A.
• Ellisman, Mark H.
• Gonzales, Cesar Nava
• Madany, Matthew
• McKaughan, Quintyn
• Su, Chih-Ying
• Tsang, Tin Ki

Drosophila antennae were prepared as described in Tsang et al. After cryofixation by high-pressure freezing and freeze-substitution in a cocktail containing uranyl acetate and glutaraldehyde, cryofixed antennae were rehydrated gradually at ice temperature. Rehydrated samples were then subjected to DAB labeling reaction and stained using a high-contrast en bloc staining protocol. Next, the samples were dehydrated for resin infiltration and embedded in Durcupan epoxy, followed by imaging with X-ray microscopy and then serial block-face scanning electron microscopy.

The antenna was imaged using a Zeiss Gemini scanning electron microscope equipped with a Gatan 3View2XP and OnPoint backscatter detector. Images were acquired at 2.5 kV accelerating voltage with a 30 μm condenser aperture and 2.5 μsec dwell time; Z step size was 50 nm; pixel size was 3.85 nm; raster size was 20k x 13.75k; Z dimension was 999 sections, and variable pressure under nitrogen. Volume was aligned using cross correlation, segmented, and visualized using IMOD.

Item type: recorded image
Imaging mode: serial block face SEM (SBFSEM)

• Cell Image Library Group ID: 20079

• Drosophila

• Dendrite
• Mitochondria
• Olfactory receptor neuron
• Sensillum lumen
• Vacuole

• data
• image
• text

• No linguistic content; Not applicable

Samplenumber: 54607

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL54607

Reference

• Cesar Nava Gonzales, Quintyn McKaughan, Eric A Bushong, Kalyani Cauwenberghs, Renny Ng, Matthew Madany, Mark H Ellisman, Chih-Ying Su (2021) Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster eLife 10:e69896. https://doi.org/10.7554/eLife.69896
• Cesar Nava Gonzales, Quintyn McKaughan, Eric A Bushong, Kalyani Cauwenberghs, Renny Ng, Matthew Madany, Mark H Ellisman, Chih-Ying Su. Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster. bioRxiv 2021.04.28.441861. https://doi.org/10.1101/2021.04.28.441861

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2024-01-12