CIL:37080, Xenopus laevis, CNS neuron (sensu Vertebrata)
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- Cite This Work
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Bestman, Jennifer; Cline, Holly (2022). CIL:37080, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00001ZD
- Description
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This is IMAGE #5 (hour 6) of a developing neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.
The cell was imaged daily over 3 days and shows the development of the dendritic arbor and axon over time. This is a second image of this cell at this timepoint.
The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope. - Date Issued
- 2022
- Researchers
- Methods
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Specifications of the custom built two-photon microscope, including the laser sources, signal amplification, PMT specifications, and YFP filter sets are described in:
Ruthazer ES, Li J, Cline HT. 2006. Stabilization of axon branch dynamics by synaptic maturation. J Neurosci 26(13):3594-3603.
The methods of cell transfection with electroporation can be found here:
Bestman JE, Ewald RC, Chiu S-L, Cline HT. 2006. In vivo single-cell electroporation for transfer of DNA and macromolecules. Nat Protocols 1(3):1267-1272.
Further explanation can be found here:
Bestman JE, Cline HT. 2008. The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo. Proc Natl Acad Sci U S A 105(51):20494-20499.
Bestman JE, Cline HT. 2009. The Relationship between Dendritic Branch Dynamics and CPEB-Labeled RNP Granules Captured in Vivo. Front Neural Circuits 3:10. - Technical Details
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Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: multi-photon microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Series
- Scientific Name
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- Identifier
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Samplenumber: 37080
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL37080
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-01-30