Withers, Ginger (2021). CIL:701, Rattus, multipolar neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J07081GB

Time lapse recording showing the development of neuron polarity in a hippocampal neuron growing in vitro. This recording captures not only the formation of the axon (the longest process, oriented downward in the last frame), but also accelerated development and outgrowth of the dendritic arbor due to treatment with BMP-7, a growth factor that has been found to selectively promote dendritic development in these cells [as described in Withers et al., (2000) PMID: 10651865].

The coverslip containing neurons was mounted in a sealed temperature controlled chamber (Warner Instruments) in phenol-free Minimal Essential Medium supplemented with N2, pyruvate, and 30 ng/ml human recombinant BMP-7. Recording was made using an inverted Leica DMIRBE microscope, 40X PL Fluotar, 0.75 N.A. objective, Princeton Instruments MicroMax camera, and MetaMorph software. Images were acquired every 5 minutes, beginning 5 hours after plating, and continuing for over 19 hours.

• 2021

• Withers, Ginger

Relation to intact cell: living tissue; dispersed cells in vitro
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Processing history: unprocessed raw data

• Rattus

• Axon
• Axonal growth cone
• Dendrite
• Dendritic branch
• Dendritic growth cone
• Multipolar neuron

• Dendrite development
• Dendrite morphogenesis
• Establishment or maintenance of cell polarity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 701

Doi: https://doi.org/10.6075/J07081GB

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL701

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=10651865
• Withers GS, Higgins D, Charette M, Banker G. Bone morphogenetic protein-7 enhances dendritic growth and receptivity to innervation in cultured hippocampal neurons. Eur J Neurosci. 2000 Jan;12(1):106-16. https://doi.org/10.1046/j.1460-9568.2000.00889.x

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2022-08-12