Ip, Wallace (2021). CIL:832, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WW7GZG

Purified rabbit skeletal muscle myosin in 0.6M KCl was deposited onto mica flakes, quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 11 degrees and visualized in a JEOL 100CX2 transmission electron microscope at 100 kev.

Images were recorded on Kodak 4489 film at 100,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED.

Note the classic appearance of the individual molecules with two globular heads (myosin subfragment 1) and the long rod-like tail.

RIGHT image of a stereo pair.

• 2021

• Ip, Wallace

Preparation: unfixed tissue; freeze_fracture/freeze_etch
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons; widefield illumination; wide-field detection
Parameter imaged: electron density
Source of contrast: electron density; elevation
Visualization methods: shadowing and plating
Processing history: unprocessed raw data

• Cell Image Library Group ID: 1847

• Oryctolagus cuniculus

• Cytoskeleton
• Skeletal muscle cell

• ATPase activity, coupled to movement of substances
• Skeletal muscle contraction

• data
• image

• No linguistic content; Not applicable

Samplenumber: 832

Doi: https://doi.org/10.6075/J0WW7GZG

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL832

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2022-08-12