Shroff, Hari; Galbraith, Catherine G.; Galbraith, James A.; White, Helen; Gillette, Jennifer; Olenych, Scott; Davidson, Michael W.; Betzig, Eric (2021). CIL:38604, Homo sapiens, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PK0DT7

This total internal reflection (TIRF) image of unconverted tdEos-paxillin (green) and PsCFP20-zyxin (red) demonstrates that these two proteins are almost completely colocalized when observed with diffraction limited optics. The true localization pattern is shown in the corresponding photoactivation localization microscopy (PALM) image, CIL 38602. Note the differential interference contrast (DIC) image corresponding to the same image field is also available as CIL 38603. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 4 in PNAS U S A. 2007 Dec 18;104(51):20308-13.

• 2021

• Betzig, Eric
• Davidson, Michael W.
• Galbraith, Catherine G.
• Galbraith, James A.
• Gillette, Jennifer
• Olenych, Scott
• Shroff, Hari
• White, Helen

Preparation: formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: TIRF (total internal reflection)
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: PsCFP2; tdEos
Processing history: unprocessed raw data
Data qualification: Raw

• Cell Image Library Group ID: 9553

• Homo sapiens

• Fibroblast
• Focal adhesion
• Paxillin
• Zyxin

• Focal adhesion assembly
• Foreskin fibroblast
• Molecular organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 38604

Doi: https://doi.org/10.6075/J0PK0DT7

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL38604

Reference

• Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes. Hari Shroff, Catherine G. Galbraith, James A. Galbraith, Helen White, Jennifer Gillette, Scott Olenych, Michael W. Davidson, and Eric Betzig. December 18, 2007, 104 (51) 20308-20313. https://doi.org/10.1073/pnas.0710517105
• Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 Dec 12. Erratum in: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15220. https://www.ncbi.nlm.nih.gov/pubmed/?term=18077327

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2022-08-12