Thoma, Claudio R.; Matov, Alexandre; Gutbrodt, Katrin L.; Hoerner, Christian R.; Smole, Zlatko; Krek, Wilhelm; Danuser, Gaudenz (2021). CIL:31263, Homo sapiens, epithelial cell, renal cell carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0G73CBC

Immunofluorescence images used to investigate stabilization of microtubules (MTs) by Von Hippel-Lindau tumor suppressor protein (pVHL). VHL-deficient RCC-4 cells with empty vector have a lower alpha-tubulin (MTs; green) signal relative to GSK-3beta (red; an internal fluorescence control), compared with RCC-4 cells expressing pVHL30, suggesting that VHL stabilizes MTs. RCC-4 cells were infected with a retroviral vector to express pVHL30, fixed in 3% paraformaledehyde and permeabilized in 0.2% Triton X-100. Primary antibodies used were: monoclonal anti-GSK3beta and polyclonal anti-alpha tubulin. Secondary antibodies used were: anti-rabbit FITC and anti-mouse Texas-Red. Stacks of 5 z planes were acquired with a Leica SP2 system using the 63x 1.4NA, Oil, HCX Plan-Apo Objective and the distance between z planes is 1 micron. This stack corresponds to J Cell Biol. 2010. 190: 991-1003--Fig 1C (left panels) and the images of RCC-4 expressing pVHL30 are in Fig 1C (right panels) and CIL# 31263.

• 2021

• Danuser, Gaudenz
• Gutbrodt, Katrin L.
• Hoerner, Christian R.
• Krek, Wilhelm
• Matov, Alexandre
• Smole, Zlatko
• Thoma, Claudio R.

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody; Fluorescein (FITC); TexasRed
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 8073

• Homo sapiens

• Axin-APC-beta-catenin-GSK3B complex
• Cytoskeleton
• Epithelial cell
• Microtubule
• Protein complex
• Renal cell carcinoma

• ATP binding
• GTPase activity
• Intracellular signal transduction
• Kinase activity
• Peptidyl-serine phosphorylation
• Protein binding
• Protein polymerization
• RCC4
• Structural molecule activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 31263

Doi: https://doi.org/10.6075/J0G73CBC

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL31263

Reference

• J Cell Biol. 2010. 190: 991-1003. https://www.ncbi.nlm.nih.gov/pubmed/?term=20855504

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

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2022-08-12