CIL:36650, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:36650, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0X9297J
- Description
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Except that the nuclear envelope remains intact, micronuclear mitosis occurs in a normal way passing through the four stages of prophase, metaphase, anaphase and telophase. In this example a micronucleus was sectioned in early anaphase and shows condensed daughter chromosomes at each pole. Chromosomes are separated by a large bundle of microtubules making up an intranuclear spindle. The separation spindle will become a very long thin tube (see Fig.20 in Allen, Ueno and Fok, J. Protozool. 35:400-407, 1988). The image shown here has very low contrast because the cell was taken from a culture in which a 15 minute treatment in ammonium chloride was used to see what effect this base would have on its acidic compartments. The cell was then incubated in diaminobenzidine. As usual the cristae of mitochondria become electron-opaque following this treatment. TEM taken on 6/11/86 by R. Allen with Zeiss 10A operating at 80kV. Neg. 4,000X. Bar = 1µm.
- Date Issued
- 2021
- Researcher
- Methods
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Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed - Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 36650
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36650
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12