Ellisman, Mark; Larson, Stephen; Maynard, Sarah; Bushong, Eric; Martone, Maryann; Berlanga, Monica (2021). CIL:40222, Mus musculus, astrocyte of the hippocampus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04B31GZ

Single computed slice through a tomographic reconstruction of the cell body of a protoplasmic astrocyte in a 0.5 um thick section from the hippocampus of a 1 month old male mouse, imaged with intermediate voltage electron microscopy. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. The reconstruction is the 5th in a series of 26 serial reconstructions through the cell soma. All 26 images can be found in this image group within the Cell Library, and the complete reconstruction can be viewed in the Cell centered database, accession # 7503.

• 2021

• Berlanga, Monica
• Bushong, Eric
• Ellisman, Mark
• Larson, Stephen
• Martone, Maryann
• Maynard, Sarah

Detailed methods can be found at the CCDB link provided. Briefly, one month old male mouse (C57BL/6NHsd) was perfused with Ringer's solution for 2 minutes followed by 2% glutaraldehyde and 2% paraformaldehyde in 0.15M sodium cacodylate buffer, pH 7.4. Further dissection of tissue was followed with postfixation using 1% OsO4, additional staining using 2% uranyl acetate, processed for embedding in durcupan ACM resin, and sectioned at a thickness of 5µm thick. Serial tomograms were produced from single tilt images using a JEOL4000, with 8k x 8k CCD camera, 5000X, 400.0 keV).

Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: osmium tetroxide; lead salt
Data qualification: Processed

• Cell Image Library Group ID: 11119

• Mus musculus

• Astrocyte of the hippocampus
• Cell body

• Central nervous system structural organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40222

Doi: https://doi.org/10.6075/J04B31GZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40222

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0NZ87G6

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2022-08-12