CIL:35214, Mus musculus, fibroblast
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- Collection
- Cite This Work
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Christensen, Søren T.; Schneider, Linda; Cammer, Michael (2021). CIL:35214, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GT5M29
- Description
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Time lapse movie of mouse embryonic fibroblasts in culture imaged at 30 second intervals by phase contrast microscopy. A micropipette is positioned near the cells to deliver either a growth factor or control buffer. In this sequence when a growth factor is added the cells activate a ring of ruffles that begin at the periphery and converge most likely at the site of the primary cilium followed by whole cell motility towards the pipette tip.
- Date Issued
- 2021
- Researchers
- Methods
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These data were collected as research which led to the published article Cell Physiol Biochem 2010;25:279-292. Microscopy was performed with a 20X planapo phase contrast objective on an Olympus IX 70 microscope with a SensicamHQ cooled CCD camera and a heated stage insert set to 37 degrees C. Cells were grown and serum starved for 48 h in a coverslip bottom MatTek dish. Small amounts of PDGF-BB were continuously ejected from the micropipette to create a gradient in the vicinity of growth-arrested wt MEFs. Cell movement was monitored with time lapse video microscopy, taking images at 30 second intervals. A Femtojet Micromanipulator 5171 (Eppendorf-Brinkman Instruments) and a pump (model Femtojet; Eppendorf) were used to control the position of the micropipette and the pressure
required for the chemoattractant flow. Femptotip II micropipettes were positioned within 1 μm
of the cover slip and pressure set at 30 to 45 hPa. Spatial scale is approximate. - Technical Details
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Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements - Series
- Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 35214
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35214
- Cell Physiol Biochem 2010;25:279-292. https://www.ncbi.nlm.nih.gov/pubmed/?term=20110689
- Journal Article: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924811/
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- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12