CIL:40952, Rattus, hippocampal neuron
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- Collection
- Cite This Work
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Korobova, Farida; Svitkina, Tatyana (2021). CIL:40952, Rattus, hippocampal neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06M35N7
- Description
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Colorized transmission electron micrograph of a platinum replica showing the cytoskeletal organization of stubby dendritic spines in extracted hippocampal neurons after 14 DIV. Unlabeled inset shows the smaller version of the corresponding main panel with axons color-coded in purple, dendrites in yellow, and spines in cyan. Yellow box indicates the enlarged panel shown on the bottom of the image. This box shows branched actin filaments (cyan) in the spine bases associate with dendritic microtubules (red). Bar, 0.2 μm. Image corresponds to Figure 3b in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel (this image) is available as CIL 40952. The uncolorized image is available as CIL 40804, and the insert is available as CIL 40810.
- Date Issued
- 2021
- Researchers
- Methods
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Please refer to referenced article for details on cell extraction and processing for electron microscopy.
- Technical Details
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Preparation: glutaraldehyde fixed tissue; permeabilized tissue; critical_point dried specimen
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: colorized - Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 40952
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40952
- Korobova F, Svitkina T. Molecular architecture of synaptic actin cytoskeleton in hippocampal neurons reveals a mechanism of dendritic spine morphogenesis. Mol Biol Cell. 2010 Jan 1;21(1):165-76. https://doi.org/10.1091/mbc.e09-07-0596
- Mol Biol Cell. 2010 Jan 1;21(1):165-76. Epub 2009 Nov 4. https://www.ncbi.nlm.nih.gov/pubmed/?term=19889835
Source data
Reference
- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12