Ammar, El-Desouky; Hogenhout, Saskia A.; Nault, L.R. (2021). CIL:25364, Maize mosaic virus, Peregrinus maidis, secretory cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RF5SX8

Electron micrograph of Maize mosaic virus (MMV, Rhabdoviridae) in the accessory salivary gland of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. MMV buds through nuclear membranes and accumulates in perinuclear space in cells of the accessory salivary gland. The accompanying image in this image group includes labels and scale bar.

Methods: Dissected insect organs were processed for TEM by fixation in glutaraldehyde and osmium tetroxide and embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM.

Associated references:
Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85.
Ammar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41.

• 2021

• Ammar, El-Desouky
• Hogenhout, Saskia A.
• Nault, L.R.

Preparation: osmium tetroxide fixed tissue; glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: detection of electrons; illumination by electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 7977

• Maize mosaic virus
• Peregrinus maidis

• Host cell perinuclear region of cytoplasm
• Nuclear membrane
• Nucleus
• Secretory cell

• Interspecies interaction between organisms
• Viral transmission by vector

• data
• image

• No linguistic content; Not applicable

Samplenumber: 25364

Doi: https://doi.org/10.6075/J0RF5SX8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25364

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12