Harding, Mark (2021). CIL:47372, Staphylococcus aureus, neutrophil, endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0SX6BSM

Visualization of neutrophil recruitment in capillaries proximal to agarose beads. (Figure 5 - Venular data)

• 2021

• Harding, Mark

Mice were anesthetized with ketamine/xylazine, and then subcutaneous tissue on a dorsal flap was exteriorized. GFP staphylococcus aureus beads were inserted. Antibodies were injected into the vasculature to label neutrophils (red) and endothelial cells (blue). Blocking antibodies against the molecule Mac1 were administered after 2 hours of infection.

Relation to intact cell: intact cells-in vivo imaging
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Source of contrast: antibody labelling
Processing history: Converted from volocity to .avi

• Cell Image Library Group ID: 16614

• Staphylococcus aureus

• Endothelial cell
• Neutrophil

• Blocking of A4 and CD18 integrins
• Immune cell recruitment

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 47372

Doi: https://doi.org/10.6075/J0SX6BSM

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL47372

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2022-08-12