Galbraith, Catherine G.; Galbraith, James A. (2021). CIL:7053, Mus musculus, permanent cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J03J3C0K

NIH 3T3 cell expressing EGFP-Lifeact (a small genetically expressed probe that binds to actin and is derived from the first 17 aa of Abp140) imaged with structured illumination. Structured illumination produces images that have a lateral resolution of approximately 100 nm, or twice the diffraction limited 200 nm resolution of conventional fluorescent microscopy techniques.

There are three related images in this folder. This file is the raw data. The 5 positions of the illumination grid pattern are visible in the 10 slices of the z-series. The structure of this file is that all 150 images appear as a horizontally incrementing sequence. The second file is a z-series of six of the reconstructed structured illumination images. The third file contains the maximum projection image of the second file. The second and third files each have 4 channels of data to compare image resolution: channel 1 - structured illumination, channel 2 - wide field, channel 3 - deconvolved wide field, and channel 4 - filtered deconvolved wide field.

Images were collected with a Carl Zeiss SIM microscope using a 63 X 1.4 Plan Apo objective, an Andor Ixon camera with 150 ms exposures,and Zeiss filter set 2.

• 2021

• Galbraith, Catherine G.
• Galbraith, James A.

Preparation: formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: structured illumination microscopy (SIM)
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 1579

• Mus musculus

• Actin cytoskeleton
• Permanent cell line cell

• Actin cytoskeleton organization
• NIH/3T3

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7053

Doi: https://doi.org/10.6075/J03J3C0K

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7053

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2022-08-12