Allen, Richard (2021). CIL:40553, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0VT1QVR

The nascent phagosome membrane has a dense coat of acidosomes docked at its cytosolic surface well before the nascent vacuole pinches off the cytopharynx. This micrograph is a quick-freeze deep-etch image of a transverse section through the posterior portion of the buccal cavity with a nascent vacuole bulging from the cytopharynx. Living cells were spun down in a centrifuge and a slurry of cells was mounted on a copper support before slam freezing the living cells against a highly polished copper surface. Some paramecia had been disrupted and this cell was feeding on the particulate organelles of the disrupted cells. Thus the nascent vacuole contains these particles amassed against its luminal surface. The cilia in the buccal cavity obviously efficiently sweep the particles against the dorsal posterior surface. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 4,000X. Bar = 1µm. Published in J. Cell Sci. 106:411-422, 1993. Adapted with permission.

• 2021

• Allen, Richard

The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: freeze_fracture/freeze_etch
Relation to intact cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: electron density
Parameter imaged: elastic scattering of electrons; electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: transmission electron microscopy (TEM)
Processing history: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 11123

• Paramecium multimicronucleatum

• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Oral apparatus
• Pre-autophagosomal structure membrane
• Vesicle membrane

• Membrane to membrane docking
• Oral apparatus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40553

Doi: https://doi.org/10.6075/J0VT1QVR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40553

Reference

• Allen RD, Ma L, Fok AK. Acidosomes: recipients of multiple sources of membrane and cargo during development and maturation. J Cell Sci. 1993 Sep;106 ( Pt 1):411-22. https://doi.org/10.1242/jcs.106.1.411
• J Cell Sci. 1993 Sep;106 ( Pt 1):411-22.
• Pubmed article: https://pubmed.ncbi.nlm.nih.gov/8270639

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12