Lauzon, Dee; Lancelle, Sue; Rice, Marian (2021). CIL:39768, Mus musculus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TM79DV

Transmission electron micrograph of coated vesicles in mouse cardiac tissue. Coated vesicles have the materials bound to the receptor molecules incorporated within their membranes allowing the vesicle to pick up and drop off material continuously re-use, this is called receptor-mediated endocytosis.

• 2021

• Lancelle, Sue
• Lauzon, Dee
• Rice, Marian

Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in 0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr's resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV.

Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment; ethanol dehydration
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10969

• Mus musculus

• Coated vesicle
• Mitochondrion
• TS25,ventricle

• Vesicle-mediated transport

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39768

Doi: https://doi.org/10.6075/J0TM79DV

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39768

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2022-08-12