Zhang, Haitao; Cao, Xiaolei; Tang, Mei; Zhong, Guoxuan; Si, Yuan; Li, Haidong; Zhu, Feifeng; Liao, Qinghua; Li, Liuju; Zhao, Jianhui; Feng, Jia; Li, Shuaifeng; Wang, Chenliang; Kaulich, Manuel; Wang, Fangwei; Chen, Liangyi; L, Li; Xia, Zongping; Liang, Tingbo; Lu, Huasong; Feng, Xin-Hua; Zhao, Bin (2022). CIL: 53824. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0SB45FF

OTHER family: The human kinome comprises 538 kinases playing essential cellular functions by catalyzing protein phosphorylations. Systematic annotation of subcellular spatial distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 compartments of the cell. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a unified condition. KA revealed kinase family-enriched subcellular localizations, and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other unique structures. This resource provides the first snapshot of the kinome under a unified condition, and is a key step toward comprehensive understanding of the spatial and temporal dynamics of the kinome in physiological and disease states.

• 2022

• Cao, Xiaolei
• Chen, Liangyi
• Feng, Jia
• Feng, Xin-Hua
• Kaulich, Manuel
• L, Li
• Li, Haidong
• Li, Liuju
• Li, Shuaifeng
• Liang, Tingbo
• Liao, Qinghua
• Lu, Huasong
• Si, Yuan
• Tang, Mei
• Wang, Chenliang
• Wang, Fangwei
• Xia, Zongping
• Zhang, Haitao
• Zhao, Bin
• Zhao, Jianhui
• Zhong, Guoxuan
• Zhu, Feifeng

Kinome Atlas documented representative images for 456 kinases expressed in HeLa cells, and visualized by immunofluorescence staining of the epitope tag. Nuclei were stained with DAPI. Two representative views from confocal microscopy were collected for each kinase. Also, organelle markers were co-stained for some kinases to indicate specific localization.

Visualization methods: confocal microscopy

• Cell Image Library Group ID: 20066

• Homo sapiens

• Cell

• HeLa
• Phosphorylation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 53824

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL53824

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-11-18