Huranova, M.; Ivani, I.; Benda, A.; Poser, I.; Brody, Y.; Hof, M.; Shav-Tal, Y.; Neugebauer, K.M.; Stanek, D. (2021). CIL:26592, Homo sapiens, cervical epithelium. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0V123N2

DRB-treated HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein U1-70K and imaged witb a PicoQuant MicroTime 200 confocal microscope using an Olympus 60x 1.2NA water immersion objective lens. DRB inhibition of transcription results in enlargement and rounding up of splicing factor compartments in which the U1-70K is enriched. From Fig 2C in Huranova et al. 2010 J Cell Biol 191:75-86.

• 2021

• Benda, A.
• Brody, Y.
• Hof, M.
• Huranova, M.
• Ivani, I.
• Neugebauer, K.M.
• Poser, I.
• Shav-Tal, Y.
• Stanek, D.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: line averaging
Data qualification: Processed

• Cell Image Library Group ID: 10294

• Homo sapiens

• Cervical epithelium
• Pre-mRNA
• SnRNP
• Spliceosomal complex

• HeLa
• RNA metabolic process
• RNA splicing

• data
• image

• No linguistic content; Not applicable

Samplenumber: 26592

Doi: https://doi.org/10.6075/J0V123N2

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL26592

Reference

• M Huranova et al. 2010 J Cell Biol 191:75-86 https://doi.org/10.1083/jcb.201004030

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2022-08-12