LaFountain, James R.; Oldenbourg, Rudolf (2021). CIL:12024, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04X56VK

Movie of meiosis I (diakinesis through telophase)in primary spermatocytes of the crane-fly Nephrotoma suturalis that have been experimentally flattened. Flattening prevents completion of cytokinesis.
Time-lapse DIC microscopy using a Nikon
Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 2 min for 179 min using a QImaging Retigo EXi CCD camera. A movie of the time series is included in this grouped set.

• 2021

• LaFountain, James R.
• Oldenbourg, Rudolf

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: differential interference contrast microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: differences in refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 3780

• Nephrotoma
• Nephrotoma suturalis

• Nuclear chromosome
• Primary spermatocyte
• Spindle

• Male meiosis chromosome segregation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12024

Doi: https://doi.org/10.6075/J04X56VK

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12024

Reference

• LaFountain JR, et al. (2004) Mol Cell Biol 15, 5724-5732 https://doi.org/10.1091/mbc.e04-06-0524
• LaFountain JR, Oldenbourg R (2004) Mol Biol Cell 15, 5346-5355. https://www.ncbi.nlm.nih.gov/pubmed/?term=15385630

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2022-08-12