Ris, Hans (2021). CIL:10424, Gallus gallus, erythrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0639NZ5

Nucleated erythrocytes from chicken were spread on water, the dispersed chromatin picked up on carbon-formvar grids, fixed with paraformaldehye, critical point dried and shadowed with platinum. Images were obtained with the Wisconsin high voltage TEM at 1MEV using darkfield illumination. For this micrograph, the grid was tilted to 54 degrees. A similar micrograph tilted to 43 degrees providing a stereo pair with an oblique 3D view of the tangled and irregular chromatin fibers is grouped with this image. The combination of shadowing and darkfield illumination gives a quasi-negative stain effect revealing nucleosomes in some fibers.

• 2021

• Ris, Hans

Preparation: formaldehyde fixed tissue; critical_point dried specimen
Relation to intact cell: spread preparation
Item type: film
Imaging mode: transmission electron microscopy (TEM); darkfield illumination
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data

• Cell Image Library Group ID: 2877

• Gallus gallus

• Erythrocyte
• Nuclear chromatin

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10424

Doi: https://doi.org/10.6075/J0639NZ5

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10424

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2022-08-12