CIL:40114, Drosophila melanogaster, embryonic cell
CIL source metadata (JSON)
File Size |
|
File Format |
|
- Collection
- Cite This Work
-
Ellisman, Mark; Sosinsky, Gina; Jones, Ying (2021). CIL:40114, Drosophila melanogaster, embryonic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0JS9PSB
- Description
-
Electron micrograph of a cultured Drosophila DL1 cell infected with flock house virus, prepared by high pressure freezing followed by freeze substitution. This cell was prepared as part of an experiment to investigate different protocols for high pressure freezing. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
- Date Issued
- 2021
- Researchers
- Methods
-
Cell pellets were directly placed into brass planchettes that then were loaded in to the HPM 010 high pressure freezer and fast frozen.
Freeze substitution: After freezing, samples (2) and (3) were placed into a Leica EM AFS Freeze substitution (FS) machine (Leica Microsystems, Bannockburn, IL) and incubated at -90 deg C for 24 hours in 0.1 percent tannic acid in acetone. Samples were washed three times with cold acetone (cooled to -90 degrees C) over 5 minutes, and placed in 1 percent OsO4 and 0.1% UA in cold acetone for 72 hours and held at -90 degrees C. After slowly warming to room temperature at 5 degrees C per hour, the specimens were rinsed in pure acetone three times (10 min. at room temperature). Infiltration and embedding in Durcupan resin was subsequently performed at room temperature. Images were acquired using a JEOL4000EX IVEM, magnification: 30000.0; accelerating voltage: 80.0 keV. - Technical Details
-
Preparation: acetone fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Parameter imaged: electron density
Data qualification: Processed - Series
- Scientific Name
- Anatomy
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Identifier
-
Samplenumber: 40114
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40114
- Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J07H1JD5
Source data
Previous version
- License
- Copyright
-
Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
-
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12