Ammar, El-Desouky; Hogenhout, Saskia A. (2021). CIL:12585, Spiroplasma kunkelii, Dalbulus maidis, prokaryotic cell, muscle cell, fat cell, secretory cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0D50KNF

A confocal laser scanning micrograph of a paraffin section in the head and thorax of leafhopper vector D. maidis, showing corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates (green fluorescence) in muscle fibers (mf), salivary glands (sg) and fat tissue (ft), close to the subesophageal ganglion (seg) and compound ganglion (cgm). Whole-mount organs were labeled with primary CSS antibodies then with secondary antibodies (Alexa Fluor-488, green), and stained with the nuclear stain propidium iodide (red). CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. For additional information refer to Ammar and Hougenhout(2005) Ann. Entomol. Soc. Am. 98(6): 820Ð826.

• 2021

• Ammar, El-Desouky
• Hogenhout, Saskia A.

Preparation: formaldehyde fixed tissue; permeabilized tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488; propidium iodide
Data qualification: Processed

• Dalbulus maidis
• Spiroplasma kunkelii

• Fat cell
• Muscle cell
• Nucleus
• Prokaryotic cell
• Secretory cell

• Pathogenesis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12585

Doi: https://doi.org/10.6075/J0D50KNF

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12585

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2022-08-12