Allen, Richard (2021). CIL:39722, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0R78DJV

The acidosome-like vesicle has at least 2 enclosing membranes or one membrane indented back on itself and an internal electron-opaque tongue-like droplet that is continuous with the enclosing membranes at one end of the vesicle and may arise by invagination of these membranes into the vesicle’s interior. Such vesicles do not resemble lysosomes of any other organism, including Paramecium. Lysosome membranes should be thicker and be coated by a heavy glycocalyx and exhibit latency. There is no glycocalyx seen here. TEM taken on 8/15/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 10657

• Tetrahymena pyriformis

• Acidosome
• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Organelle membrane

• Digestive system process

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39722

Doi: https://doi.org/10.6075/J0R78DJV

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39722

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2022-08-12