CIL:36780, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
CIL source metadata (JSON)
File Size |
|
File Format |
|
- Collection
- Cite This Work
-
Allen, Richard (2021). CIL:36780, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J07H1H7H
- Description
-
Sections show the origin of one cytopharyngeal ribbon of 12 microtubules from the nodes and show the tight curve the ribbon follows over the cytostomal cord. These ribbons move discoidal vesicles, acidosomes and carrier vesicles along one of their surfaces. Carrier vesicles are most abundant under the quadrulus where they arise from early endosomes and where the highest concentration of parasomal sacs and early endosomes occurs within the cell. These vesicles rapidly fuse with acidosomes. The acidosomes then dock at the membrane of the nascent phagosome. TEM taken on 4/10/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,750X. Bar = 0.5µm. Published in Poste and Nicolson (eds.) Membrane Fusion, Elsevier/North Holland Biomed. Press, p.735, 1978 and in J. Cell Biol. 1974. 63:904-22. Adapted with permission.
- Date Issued
- 2021
- Researcher
- Methods
-
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39164) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
-
Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; osmium tetroxide; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed - Series
- Scientific Name
- Anatomy
- Topics
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Identifier
-
Samplenumber: 36780
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36780
- Allen RD. Food vacuole membrane growth with microtubule-associated membrane transport in Paramecium. J Cell Biol. 1974 Dec;63(3):904-22. https://doi.org/10.1083/jcb.63.3.904
- Pubmed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=4373478
Source data
Reference
- License
-
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
-
Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
-
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12