Svitkina, Tatyana M.; Borisy, Gary G. (2021). CIL:24811, Xenopus laevis, keratocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0C8282K

Localization of Xenopus ADF/cofilin (XAC) to posterior regions of depolymerization-resistant actin brush. Electron micrograph of lamellipodia of Xenopus keratocytes after latrunculin a treatment ( 0.25 μM for 10 min) and subsequent staining with XAC antibody. Image correspond to Figure 10 c from J Cell Biol. 1999 May 31;145(5):1009-26.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Preparation: permeabilized tissue; glutaraldehyde fixed tissue; polyethylene glycol
Relation to intact cell: dispersed cell in vitro
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: optical path length gradient
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating; primary antibody plus labeled secondary antibody; platinum replica; immuno gold
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9875

• Xenopus laevis

• Actin cytoskeleton
• Keratocyte
• Lamellipodium
• Xenopus ADF/cofilin

• Cellular localization
• Latrunculin a treatment

• data
• image

• No linguistic content; Not applicable

Samplenumber: 24811

Doi: https://doi.org/10.6075/J0C8282K

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL24811

Reference

• J Cell Biol. 1999 May 31;145(5):1009-26. https://www.ncbi.nlm.nih.gov/pubmed/?term=10352018

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2022-08-12