Clarke, Margaret; Silverman, Philip (2021). CIL:7322, Escherichia coli, prokaryotic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J07P8XD3

This is one of four related images of F-pili (conjugative pili encoded by the F plasmid of E. coli) dynamics by live-cell imaging. There are 2 movie (.avi) files and 2 files (.tiff) from the movies. The movies show that F-pili normally undergo cycles of extension and retraction in the absence of any obvious triggering event such as contact with a recipient cell.

Retraction of an F-pilus. E. coli strain HfrH expressing the red fluorescent marker DsRed Express were mixed with R17 bacteriophage labeled with Alexa 488 (green); R17 bacteriophage (NCBITaxon:12026) bind to the sides of F-pili.

Samples were imaged by using a Zeiss LSM 510 confocal microscope with a Plan-Apochromat 63 × 1.4 N.A. DIC objective. Alexa-488 was excited with the 488-nm line of an argon laser with a 505–530-nm filter for emission, and DsRed was excited with the 543-nm line of a HeNe laser, with a 560-nm long pass filter for emission. An HFT UV/488/543 beam splitter was used. Images were collected in a single focal plane at 2 s intervals and accompanying movies (.avi) are playing at 10fps.

• 2021

• Clarke, Margaret
• Silverman, Philip

Preparation: living tissue
Relation to intact cell: living tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label; distribution of a specific protein
Visualization methods: DsRed-ExpressFP; Alexa Fluor 488; macromolecular probe
Processing history: unprocessed raw data

• Cell Image Library Group ID: 1456

• Escherichia coli

• Pilus
• Prokaryotic cell

• Conjugation
• Pilus assembly
• Pilus retraction

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7322

Doi: https://doi.org/10.6075/J07P8XD3

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7322

Reference

• Clarke M, Maddera L, Harris RL, Silverman PM. F-pili dynamics by live-cell imaging. Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17978-81. https://doi.org/10.1073/pnas.0806786105
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=19004777

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2022-08-12