CIL:39734
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- Collection
- Cite This Work
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Robinson, Philip J.; Fairall, Louise; Huynh, Van A.; Rhodes, Daniela (2021). CIL:39734. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00R9N5J
- Description
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EM images of chromatin fibers reconstituted from tandem arrays of a strong nucleosome positioning sequence flanked by different lengths of 'linker' DNA, to create nucleosome repeat lengths (NRL) from 177 bp to 237 bp. Reconstitutes had saturating amounts of purified core histones and linker histones. (A) shows the range of structures seen with glutaraldehyde fixation and negative staining, with the left panel illustrating individual fibers and the right panel longer structures consisting of end-to-end aggregates.(B)shows images of a single NRL after cryo-fixation and recorded in the frozen hydrated state. See Fig 1 in P.J. Robinson et al. 2006. EM measurements define the dimensions of the "30-nm" chromatin fiber: Evidence for a compact, interdigitated structure. Proc Natl Acad Sci USA 103:6506-6511.
- Date Issued
- 2021
- Researchers
- Methods
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See: P.J. Robinson et al. 2006. EM measurements define the dimensions of the "30-nm" chromatin fiber: Evidence for a compact, interdigitated structure. Proc Natl Acad Sci USA 103:6506-6511.
- Technical Details
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Preparation: glutaraldehyde fixed tissue; cryofixed tissue
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain; intrinsic mass distribution
Visualization methods: visualization of contiguous regions; negative staining; uranyl salt
Processing history: montage
Data qualification: Processed - Series
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 39734
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39734
- P.J. Robinson et al. 2006. EM measurements define the dimensions of the "30-nm" chromatin fiber: Evidence for a compact, interdigitated structure Proc Natl Acad Sci USA 103:6506-6511 https://www.ncbi.nlm.nih.gov/pubmed/?term=16617109
- Proceedings National Academy of sciences USA. http://www.pnas.org/content/103/17/6506.long
Source data
Reference
- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12