Svitkina, Tatyana M.; Verkhovsky, Alexander B.; Borisy, Gary G. (2021). CIL:23047, Rattus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0X92929

Association of plectin with MTs. Electron microscopy
of gelsolin-treated REF-52 cells after immunogold (10 nm) labeling for plectin. Plectin forms bridges between microtubules and intermediate filaments. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 5a from J Cell Biol. 1996 Nov;135(4):991-1007.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.
• Verkhovsky, Alexander B.

Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; tannic acid
Relation to intact cell: platinum replica
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: uranyl salt; shadowing and plating; primary antibody plus labeled secondary antibody; immunogold
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8924

• Rattus

• Fibroblast
• Intermediate filament
• Microtubule
• Plectin

• Gelsolin treatment
• REF-52

• data
• image

• No linguistic content; Not applicable

Samplenumber: 23047

Doi: https://doi.org/10.6075/J0X92929

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL23047

Reference

• J Cell Biol. 1996 Nov;135(4):991-1007 https://www.ncbi.nlm.nih.gov/pubmed/?term=8922382

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2022-08-12