Allen, Richard (2021). CIL:34613, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0J965CS

An image of the nucleoli of the macronucleus. Nucleoli have a core of DNA that contains the genes that are transcribed into ribosomal RNA. Assembly of the ribosomal subunits from this processed RNA and from proteins transported into the nucleus through the pores occurs in the nucleoli. A concentric stack of rough ER encircles a mitochondrion. TEM taken on 8/16/67 by R. Allen with Philips 200 operating at 60kV. Neg. 7,200X. Bar = 1µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:34537) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; tissue in vitreous ice embedment; microtome-sectioned tissue; monoclonal antibody
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; gold
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 10144

• Tetrahymena pyriformis

• Cell by organism
• Ciliated Protist
• Eukaryotic cell
• Eukaryotic Protist
• Macronucleus
• Nucleolus
• Nucleolus organizer region

• Macronucleus organization
• Nucleolus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 34613

Doi: https://doi.org/10.6075/J0J965CS

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34613

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12