Ammar, El-Desouky; Hogenhout, Saskia A.; Nault, L.R. (2021). CIL:25365, Maize mosaic virus, Peregrinus maidis, hemocyte (sensu Nematoda and Protostomia). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0HX1BJ3

Aggregates of maize mosaic virus (MMV, Rhabdoviridae) in cells from hemolymph of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). Antibodies to MMV identify aggregates (green/yellow fluorescence) that accumulate mainly around the nuclei in hemocytes.

Methods: Hemolymph smears were fixed in paraformaldehyde, labeled with primary antibodies to MMV, and with secondary antibodies: Alexa Fluor-488 (green), and later stained with the nuclear stain propidium iodide (red). Smears were examined with confocal laser- scanning microscope (Leica TCS SP).

Associated references:
Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85.
Ammar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41.

• 2021

• Ammar, El-Desouky
• Hogenhout, Saskia A.
• Nault, L.R.

Preparation: formaldehyde fixed tissue; permeabilized tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: primary antibody plus labeled secondary antibody; Alexa Fluor 488; propidium iodide
Processing history: recorded image
Data qualification: Raw

• Cell Image Library Group ID: 8074

• Maize mosaic virus
• Peregrinus maidis

• Hemocyte (sensu Nematoda and Protostomia)
• Host cell perinuclear region of cytoplasm
• Nucleus

• Viral transmission by vector

• data
• image

• No linguistic content; Not applicable

Samplenumber: 25365

Doi: https://doi.org/10.6075/J0HX1BJ3

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25365

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2022-08-12