Woodcock, Christopher; Miller, Gayle (2021). CIL:555, Acetabularia mediterranea, gamete. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FN15H6

At cyst formation in the 'giant' unicellular green alga Acetabularia, each cyst contains a single nucleus which subsequently undergoes several rounds of mitosis prior to gamete formation. Initially the nuclei form a syncytium which separates into individual gametes. This section shows a stage in gametogenesis where the syncytium has just started to divide into uninuclear gametes, and the formation of flagella from paired centrioles is in progress. See Woodcock and Miller, 1973. Ultrastructural features of the life cycle of Acetabularia I Gametogenesis. Proptoplasma 77, 313-319.

• 2021

• Miller, Gayle
• Woodcock, Christopher

Cysts were fixed in glutaraldehyde, post-fixed in osmium tetroxide, epoxy embedded, and thin sections stained with aqueous uranyl acetate and lead citrate. Grids were examined with a Philips 400 TEM operated at 80KV and images recorded on film.

Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10296

• Acetabularia mediterranea

• Centriole
• Chloroplast
• Chloroplast starch grain
• Gamete
• Nucleus
• Plastid thylakoid membrane

• Gamete generation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 555

Doi: https://doi.org/10.6075/J0FN15H6

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL555

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2022-08-12