CIL:30560, Drosophila melanogaster, early embryonic cell
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- Cite This Work
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Civelekoglu-Scholey, Gul; Tao, Li; Brust-Mascher, Ingrid; Wollman, Roy; Scholey, Jonathan M. (2021). CIL:30560, Drosophila melanogaster, early embryonic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0M61HVW
- Description
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A confocal Z-stack from a transgenic Drosophila embryo expressing the kinesin-14 motor Ncd-GFP during prometaphase phase 1. This image stack is original data contributing to Fig. 1 "Prometaphase spindle dynamics in Drosophila embryos: steady-state and elongation phase" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68.
- Date Issued
- 2021
- Researchers
- Methods
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Embryos expressing GFP-Ncd were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil. Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confocal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4). For these experiments, z-stacks were acquired with an Orca II CCD camera (Hamamatsu Photonics) at intervals of 0.1-0.2 µm, using exposure times of 300-600ms. For further information see J. Cell Biol. 188(1):49-68.
- Technical Details
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Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Green fluorescent proteins from Aequorea
Data qualification: Raw - Series
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- No linguistic content; Not applicable
- Identifier
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Samplenumber: 30560
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL30560
- Pubmed article: https://pubmed.ncbi.nlm.nih.gov/65089/
- Tolarová M. Hypospadias from the genetic point of view. Annotation of experimental results. Acta Chir Plast. 1976;18(3):161-3. PMID: 65089. https://www.ncbi.nlm.nih.gov/pubmed/?term=0065089
- BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/
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- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12