Liu, Allen; Schmid, Sandra L. (2021). CIL:10104, Homo sapiens, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02J6B1F

Cultured retinal pigment epithelial cells immunofluorescently labeled for transferrin receptor (TfnR) (green) and nucleus (blue). The cells were fixed in 2% PFA and 0.5% Triton X-100 for 2 minutes followed by post-fixation 4% PFA. TfnR was detected with H68.4 primary antibody and secondary FITC antibody. The nucleus was detected with DAPI staining. Images were collected on an Olympus IX-71 epifluorescence microscope using a 100X 1.4 NA objective with 4.500ms exposure for TnfR and 50ms exposure for DAPI (67nm/pixel).

• 2021

• Liu, Allen
• Schmid, Sandra L.

Preparation: formaldehyde fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody; Fluorescein (FITC)
Processing history: unprocessed raw data

• Homo sapiens

• Epithelial cell
• Nucleus
• Transferrin receptor

• ARPE-19

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10104

Doi: https://doi.org/10.6075/J02J6B1F

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10104

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12