Sun, Yuting; Ge, Yejing; Drnevich, Jenny; Zhao, Yong; Band, Mark; Chen, Jie (2021). CIL:13592, Mus musculus, myoblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RX99S1

The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. After cultured in differentiation medium for 3 days, these cells differentiated into myotubes (Green) containing multiple nuclei (Blue). The C2C12 cells stably expressing FLAG-tagged rapamycin-resistant, kinase-inactive (RR/KI) mTOR were induced to differentiation in the presence of 50nM rapamycin for 3 days. The C2C12 cells differentiated, but arrested at the nascent myotube stage.

This image is the control of different treatments in Figure 9B from JCB 189: 1157-1169, 2010. See also CIL: 13593, 13594, 13601.

• 2021

• Band, Mark
• Chen, Jie
• Drnevich, Jenny
• Ge, Yejing
• Sun, Yuting
• Zhao, Yong

The image was taken by leica DMI 4000B microscope equipped with a QImaging RETIGA Exi camera and Leica 10X/0.22 lens.

Myosin heavy chain (MHC) expressed in myotubes was labeled by MF-20 mouse antibody followed by FITC-anti-mouse IgG secondary antibody. Nuclei were stained by DAPI. MHC and nuclei were pseudocolored in green and blue, respectively.

Preparation: formaldehyde fixed tissue; permeabilized tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: compartmentalization of stain or label; distribution of epitope
Visualization methods: Fluorescein (FITC); 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody
Processing history: brightness and contrast adjusted
Data qualification: Processed;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 8288

• Mus musculus

• Muscle myosin complex
• Myoblast
• Nucleus

• C2C12
• MTOR inhibition
• Myoblast fusion

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13592

Doi: https://doi.org/10.6075/J0RX99S1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13592

Reference

• JCB 189: 1157-1169, 2010 https://www.ncbi.nlm.nih.gov/pubmed/?term=20566686

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2022-08-12