Porter, Iain M.; McClelland, Sarah E.; Khoudoli, Guennadi A.; Hunter, Christopher J.; Andersen, Jens S.; McAinsh, Andrew D.; Blow, J. Julian; Swedlow, Jason R. (2021). CIL:13379, Homo sapiens. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0F47MV5

Z-focal series of HeLa cell at metaphase stained for DNA (blue), phospho-Ser92-MAK (mitotic centromere-associated kinesin) (red), and Aurora B (green). Phosphorylation of MCAK is reduced in Bod1siRNA cells. Cells were transfected with control (this image) or Bod1 (CIL: 13378) siRNA. After 72 h, cells were treated with monastrol for 3 h and released into media containing MG132 for 1 h before fixing. Cells were stained for total mitotic centromere-associated kinesin (MCAK) population. MCAK localizes to the inner centromere in its phosphorylated form but concentrates at kinetochores in its dephosphorylated state.

• 2021

• Andersen, Jens S.
• Blow, J. Julian
• Hunter, Christopher J.
• Khoudoli, Guennadi A.
• McAinsh, Andrew D.
• McClelland, Sarah E.
• Porter, Iain M.
• Swedlow, Jason R.

Antibodies were rabbit anti-HEC1 (green) antibody (Abcam) used at 1:1,000. and anti–phospho-MCAK antibodies (red) (Andrews et al., 2004) used at 1 μg/ml, and DNA (blue). Fluorescently labeled secondary antibodies were all obtained from Jackson ImmunoResearch Laboratories. Corresponds to Fig 5D in JCB vol. 179 no. 2 187-197.

Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody
Processing history: constrained iterative deconvolution

• Cell Image Library Group ID: 11195

• Homo sapiens

• Chromosome, centromeric region
• DNA
• HEC1
• Nuclear chromosome
• Phosphorylated MCAK

• Chromosome organization
• HeLa S3
• Mitosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13379

Doi: https://doi.org/10.6075/J0F47MV5

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13379

Reference

• JCB vol. 179 no. 2 187-197 https://www.ncbi.nlm.nih.gov/pubmed/?term=17938248
• Porter IM, McClelland SE, Khoudoli GA, Hunter CJ, Andersen JS, McAinsh AD, Blow JJ, Swedlow JR. Bod1, a novel kinetochore protein required for chromosome biorientation. J Cell Biol. 2007 Oct 22;179(2):187-97. https://doi.org/10.1083/jcb.200704098

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2022-08-12