Pentecost, M; Otto, G.; Theriot, J.A.; Amieva, M.R. (2021). CIL:25359, Canis lupus familiaris, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NG4PDB

Reorganization of cells around a site of cell extrusion monitored live by time-lapse differential interference contrast microscopy. The process of extrusion began at hour 1 of observation and continued for 40 min. Monolayer was imaged on a Zeiss Axiovert 200M microscope using a 40× dry differential interference contrast microscopy (DIC) objective. Images were collected by a charge-coupled device camera (CoolSNAP-HQ, Photometrics, Tucson, AZ) and stored digitally every 10 min. Image corresponds to Fig 4 and Video 1 in PLoS Pathog. 2006 Jan;2(1):e3. Epub 2006 Jan 27.

• 2021

• Amieva, M.R.
• Otto, G.
• Pentecost, M
• Theriot, J.A.

Preparation: living tissue
Relation to intact cell: monolayer
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 6802

• Canis lupus familiaris

• Cell
• Epithelial cell

• Cell extrusion
• MDCK

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 25359

Doi: https://doi.org/10.6075/J0NG4PDB

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25359

Reference

• PLoS Pathog. 2006 Jan;2(1):e3. Epub 2006 Jan 27 https://www.ncbi.nlm.nih.gov/pubmed/?term=16446782

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2022-08-12