Yam, Patricia T.; Wilson, Cyrus A.; Ji, Lin; Hebert, Benedict; Barnhar, Erin L.; Dye, Natalie A.; Wiseman, Paul W.; Danuser, Gaudenz; Theriot, Julie (2021). CIL:12017, Hypsophrys nicaraguensis, keratocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0J67FN1

Formation of an actin axle during the maturation of polarity. During maturation of the polarized form, the circular actin bands around the cell body transitioned to an actin axle along the cell rear. Phase-contrast and AF546-phalloidin FSM time-lapse video of a cell initiating motility and maturing. 30x real time (frames were collected at 3-s intervals and are displayed at 10 frames/s). Phalloidin was conjugated to AlexaFluor546 and cells were imaged in culture media (Leibovitz's L-15 medium without phenol red supplemented with 14.2 mM Hepes, pH 7.4, 10% FBS, and 1% antibiotic-antimycotic). Time-lapse phase contrast and epifluorescent images were acquired using an inverted microscope (Diaphot-300; Nikon) with a 60× NA 1.4 oil plan-Apo objective (Nikon). Images were acquired every 3 s with a 60× oil objective. All time-lapse images were collected with a cooled back-thinned CCD camera (MicroMax 512BFT; Princeton Instruments) with a 2× optovar attached using MetaMorph software version 6 (Molecular Devices). Corresponds to Fig. S3 and video 8 from JCB 178:1207-1221, 2007.

• 2021

• Barnhar, Erin L.
• Danuser, Gaudenz
• Dye, Natalie A.
• Hebert, Benedict
• Ji, Lin
• Theriot, Julie
• Wilson, Cyrus A.
• Wiseman, Paul W.
• Yam, Patricia T.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy; speckle microscopy
Parameter imaged: elastic scattering of photons; fluorescence emission
Source of contrast: differences in amount of elastic light scattering; distribution of a specific protein
Visualization methods: Alexa Fluor 546; phalloidin; visualization of contiguous regions
Processing history: contrast, brightness, image combine
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3422

• Hypsophrys nicaraguensis

• Actin cytoskeleton
• Keratocyte

• Actin axle formation

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 12017

Doi: https://doi.org/10.6075/J0J67FN1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12017

Reference

• Yam PT, Wilson CA, Ji L, Hebert B, Barnhart EL, Dye NA, Wiseman PW, Danuser G, Theriot JA. Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. J Cell Biol. 2007 Sep 24;178(7):1207-21. https://doi.org/10.1083/jcb.200706012
• Yam PT, Wilson CA, Ji L, Hebert B, Barnhart EL, Dye NA, Wiseman PW, Danuser G, Theriot JA. Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. J Cell Biol. 2007 Sep 24;178(7):1207-21. https://www.ncbi.nlm.nih.gov/pubmed/?term=17893245

Other resource

• Fawcett, Don W. "The Cell." 2nd ed., W.B. Saunders, 1981, ISBN 0721635849. https://www.ascb.org/career-development/teaching/don-fawcetts-the-cell/

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2022-08-12