Wittmann, Torsten; Bokoch, Gary M.; Waterman-Storer, Clare M. (2021). CIL:11835, Potorous tridactylus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00P0XST

Microtubules in a control PtK1 cell at the edge of an epithelial cell island. Few pioneer microtubules rapidly grow into forming protrusions. The cell was microinjected with X-rhodamine-conjugated tubulin and observed by fluorescence microscopy. Epifluorescence
image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 1 of associated manuscript (J Cell Biol, 161:845-851, 2003).

• 2021

• Bokoch, Gary M.
• Waterman-Storer, Clare M.
• Wittmann, Torsten

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation

• Cell Image Library Group ID: 4105

• Potorous tridactylus

• Epithelial cell
• Microtubule

• Microtubule polymerization
• Pt K1 (NBL-3)

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 11835

Doi: https://doi.org/10.6075/J00P0XST

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11835

Reference

• J Cell Biol, 161:845-851, 2003. https://www.ncbi.nlm.nih.gov/pubmed/?term=12796474

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2022-08-12