Fink, Rachel; Wadsworth, Pat (2021). CIL:35202, Fundulus heteroclitus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0D21W91

This video shows the apical (outside-facing) side of epithelia cells that form confluent tissues where each cell is tightly glued to its neighbors by circumferential junctions like bands of velcro. These cells show that the cell surface is extremely dynamic, blistering and boiling with small surface protrusions, filled with actin. This video also shows these protrusions in parts of three neighboring cells, and the circumferential actin-filled ridges at the cell margins are particularly dramatic. The actin used in these protrusions is the same pool used by deep cells for individual migration, and these enveloping (EVL) cells use the same kinds of biochemical controls for polymerization (rapid growth) and depolymerization (shrinkage) of filaments.

• 2021

• Fink, Rachel
• Wadsworth, Pat

Visualizations taken from intact embryos, injected with GFP-actin plasmid at 1-cell stage. Filmed during gastrulation.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9029

• Fundulus heteroclitus

• Actin cytoskeleton
• Epithelial cell

• Cytoskeleton organization

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 35202

Doi: https://doi.org/10.6075/J0D21W91

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35202

Other resource

• Rachael Fink videos (rvideo5.htm): https://www.mtholyoke.edu/people/rachel-fink/rvideo5.htm

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

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2022-08-12