CIL:11924, Taricha granulosa, epithelial cell of lung
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- Cite This Work
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Salmon, Wendy C.; Adams, Michael C.; Waterman-Storer, Clare M. (2021). CIL:11924, Taricha granulosa, epithelial cell of lung. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09Z93H8
- Description
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Comparison of actin speckle microscopy with phalloidin staining. Primary cultures of newt lung epithelial cells were microinjected with X-rhodamine actin. Time-lapse FSM was performed on a spinning disk confocal microscope system. Light from a 50-mW Krypton-Argon ion laser (Melles Griot, OmniChrome) was delivered by a single-mode fiber optic (Point Source) to a Yokogawa spinning disk confocal scan-head (Ultra-View; PerkinElmer) on an inverted microscope (TE300 Quantum; Nikon). Excitation wavelength was selected by a filter-wheel apparatus (Sutter Instruments Co.) containing excitation filters for 488 and 568 nm (Chroma). Emission was selected by multiple bandpass dichromatic mirror and emission filter (Chroma). Images were collected by a 100x 1.4NA Plan-Apo DIC objective lens (Nikon) and captured with an Orca 2 camera (Hamamatsu). Images were collected in the ventral focal plane at 10s intervals. Following live cell imaging, cells were fixed with paraformaldehyde, permeabilized and stained with Alexa 488 phalloidin. Images were processed as follows: (1) background subtraction; (2) 3 x3 low pass filter; (3) unsharp mask filter. Video corresponds to Fig 1 and video 1 in J Cell Biol, 158:31-37, 2002.
- Date Issued
- 2021
- Researchers
- Technical Details
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Preparation: living tissue; formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: spinning disk confocal microscopy; fluorescent speckle microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine; Alexa Fluor 488; phalloidin
Processing history: background subtraction, 3X3 low pass filter, unsharp mask
Data qualification: Processed;spatialmeasurements;intensitiesquantitation - Series
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- Identifier
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Samplenumber: 11924
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11924
- J Cell Biol, 158:31-37, 2002 https://www.ncbi.nlm.nih.gov/pubmed/?term=12105180
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- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12