Svitkina, Tatyana M.; Borisy, Gary G. (2021). CIL:34897. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W66JGR

Improved visualization of actin filament branching in lamellipodia. EM of keratocyte or fibroblast lamellipodial actin network after cytochalasin D treatment (0.2 μM for 30 min or 0.5 μM for 10 min) Image corresponds to a panel in Figure 2b from J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 2b are available as CIL 34894-34901.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Preparation: permeabilized tissue; glutaraldehyde fixed tissue; phalloidin
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: shadowing and plating; platinum replica
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9875

• Actin cytoskeleton

• Actin filament organization
• Branching of actin filaments
• Cytochalasin D treatment

• data
• image

• No linguistic content; Not applicable

Samplenumber: 34897

Doi: https://doi.org/10.6075/J0W66JGR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34897

Reference

• J Cell Biol. 1999 May 31;145(5):1009-26. https://www.ncbi.nlm.nih.gov/pubmed/?term=10352018

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2022-08-12