Svitkina, Tatyana M.; Borisy, Gary G. (2021). CIL:24806, Xenopus laevis, keratocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06M35Q4

Localization of XAC (Xenopus ADF/cofilin) in Xenopus keratocytes. Fluorescence microscopy of a whole cell double stained with XAC antibody (green) and TRITC-phalloidin (red). XAC in lamellipodium is excluded from the narrow zone at the extreme leading edge. Immuno-EM with localization of XAC is shown in CIL 24807 and 24808. Image corresponds to Figure 8a-c from J Cell Biol. 1999 May 31;145(5):1009-26.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Preparation: glutaraldehyde fixed tissue; permeabilized tissue; polyethelyne glycol
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody; Tetramethyl rhodamine (TRITC); phalloidin
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9875

• Xenopus laevis

• Actin cytoskeleton
• ADF/cofilin
• Keratocyte
• Lamellipodium

• Cellular localization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 24806

Doi: https://doi.org/10.6075/J06M35Q4

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL24806

Reference

• J Cell Biol. 1999 May 31;145(5):1009-26. https://www.ncbi.nlm.nih.gov/pubmed/?term=10352018

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2022-08-12